Article

A scalable platform for efficient CRISPR-Cas9 chemical-genetic screens of DNA damage-inducing compounds

2023-06-16

Abstract excerpt

Current approaches to define chemical-genetic interactions (CGIs) in human cell lines are resource-intensive. We designed a scalable chemical-genetic screen platform by generating a DNA damage response (DDR)-focused custom sgRNA library. We performed five proof-of-principle compound screens and found that the compounds’ known modes-of-action (MoA) were enriched among the compounds’ CGIs. These scalable screens rec...

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Literature Corpus work: fed9bb04-f562-5540-8ca6-785ec6d4a953
DOI: 10.1101/2023.06.16.545157

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