Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.

Nucleic acids research - 13 Apr 2026

White Nathan, Hu Yi-Ting, Chalk John Alexander, Kurgan Gavin, Naseem Asma, Schmaljohn Ellen, Sturgeon Morgan, Cavazza Alessia, Thrasher Adrian James, Turchiano Giandomenico

Abstract excerpt

Therapeutic gene editing with designer nucleases can be compromised by undesired repair outcomes. DNA repair inhibitors are used to bias DSB repair toward HDR, but their impact on larger structural rearrangements, including large deletions and translocations, remains unclear. We quantify the mutational burden associated with end-joining inhibitor compounds. With a highly precise Cas9 nuclease, repair inhibition...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA-Activated Protein Kinase
Share to DNA End-Joining Repair

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CAST gene

Share to Gene Editing

Share to Mutation

Share to Protein Kinase Inhibitors

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 42003552
DOI: 10.1093/nar/gkag318
PMCID: PMC13092968

Genes

CAST

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.PMID 42003552Read ↗

MEGA dPCR reveals chromosomal aberrations, NHEJ precise repair, recurrent nuclease cleavage and DSB half-lifeSemantic proximity Read ↗

Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterationsSemantic proximity Read ↗

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cellsSemantic proximity Read ↗

Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment.Semantic proximity Read ↗

Mutational signature catalogue <i>(MUSIC)</i> of DNA double-strand break repairSemantic proximity Read ↗

A mutational scar-based genome-wide map of DNA double-strand break repair.Semantic proximity Read ↗

A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage.Semantic proximity Read ↗

A comprehensive genetic catalog of human double-strand break repairSemantic proximity Read ↗

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cellsSemantic proximity Read ↗

A scalable platform for efficient CRISPR-Cas9 chemical-genetic screens of DNA damage-inducing compoundsSemantic proximity Read ↗

iMUT-seq: high-resolution DSB-induced mutation profiling reveals prevalent homologous-recombination dependent mutagenesis.Semantic proximity Read ↗

TFU72 is a novel and potent DNA-PKcs inhibitor for enhancing homology-directed repair gene editingSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.