Article

A total synthetic approach to CRISPR/Cas9 genome editing and homology directed repair

2018-07-01

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR/Cas9 has become a powerful tool for genome editing in zebrafish that permits the rapid generation of loss of function mutations and the knock-in of specific alleles using DNA templates and homology directed repair (HDR). We compared synthetic, chemically modified sgRNAs to in vitro transcribed sgRNAs and demonstrate the increased activity of synthetic sgRNAs in combination with recombinan...

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Literature Corpus work: fbc7a9e3-5794-53d4-a556-6244364e77f7
DOI: 10.1101/359984

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