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Article

A fast method for analyzing essential protein mutants in human cells.

BioTechniques - 1 Feb 2017

Dietsch Frank, Donzeau Mariel, Cordonnier Agnes M, Weiss Etienne, Chatton Bruno, Vigneron Marc

Abstract excerpt

Here we developed a complementation method for the study of essential genes in live human cells using the CRISPR/Cas9 system. Proteins encoded by essential genes were expressed using a derivative of the pCEP4 compensating plasmid in combination with Cas9 endonuclease targeting of the chromosomal genes. We show that this strategy can be applied to essential genes, such as those coding for proliferating cell...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 28193152
DOI: 10.2144/000114518

Genes

PCNA
POLD2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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