Article

Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells

2025-01-23

Abstract excerpt

<h4>ABSTRACT</h4> Perturb-seq is a powerful approach to systematically assess how genes and enhancers impact the molecular and cellular pathways of development and disease. However, technical challenges have limited its application in stem cell-based systems. Here, we benchmarked Perturb-seq across multiple CRISPRi modalities, on diverse genomic targets, in multiple human pluripotent stem cells, during directed d...

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Literature Corpus work: f78a4dea-f17d-5765-94d7-77acb177eba6
DOI: 10.1101/2025.01.21.633969

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