Article

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq

2021-12-17

Abstract excerpt

A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (pooled CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functional genomic mapping but, to date, have been used at limited scales. Here, we perform genome-scale Perturb-seq targeting all expressed genes with CRISPR interference (CRI...

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Literature Corpus work: 769d3dc5-7db8-595b-83b8-fd12b645d6de
DOI: 10.1101/2021.12.16.473013

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