Article
Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq.
Cell - 7 Jul 2022
Replogle Joseph M, Saunders Reuben A, Pogson Angela N, Hussmann Jeffrey A, Lenail Alexander, Guna Alina, Mascibroda Lauren, Wagner Eric J, Adelman Karen, Lithwick-Yanai Gila, Iremadze Nika, Oberstrass Florian, Lipson Doron, Bonnar Jessica L, Jost Marco, Norman Thomas M, Weissman Jonathan S
Abstract excerpt
A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functional genomic mapping but, to date, have been used at limited scales. Here, we perform genome-scale Perturb-seq targeting all expressed genes with CRISPR interference (CRISPRi)...
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