Article

Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contexts

2025-11-14

Abstract excerpt

Multiplexed assays of variant effect (MAVEs) systematically measure variant function but have been limited to cancer cell lines rather than disease-relevant cell types. We developed saturation genome editing in human iPSCs (iPSC-SGE) to introduce variant libraries into a single allele of a target gene while programming the genetic background of the second allele, enabling variant assessment across differentiated c...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 417904ea-0d0b-5bfb-bbd3-65472ce0be11
DOI: 10.1101/2025.11.12.25340127

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contextsDOI 10.1101/2025.11.12.25340127Read

High-throughput screening of human genetic variants by pooled prime editingSemantic proximity Read

High-throughput screening of human genetic variants by pooled prime editingSemantic proximity Read

Control iPSC lines with clinically annotated genetic variants for versatile multi-lineage differentiationSemantic proximity Read

Discovering mechanisms of human genetic variation and controlling cell states at scale.Semantic proximity Read

Functional phenotyping of genomic variants using joint multiomic single-cell DNA-RNA sequencing.Semantic proximity Read

iPSCORE: A Resource of 222 iPSC Lines Enabling Functional Characterization of Genetic Variation across a Variety of Cell Types.Semantic proximity Read

Stem-cell-based functional genomics for the large-scale analysis of genetic variants.Semantic proximity Read

Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulationSemantic proximity Read

Precision Health Resource of Control iPSC Lines for Versatile Multilineage Differentiation.Semantic proximity Read

Leveraging human genetic variation to therapeutically target hundreds of genes with dominant & dispensable disease allelesSemantic proximity Read

Prime Editing Driven Functional Genomics: Bridging Genotype to Phenotype in the Post-Genomic Era.Semantic proximity Read

Scaled and Efficient Derivation of Loss of Function Alleles in Risk Genes for Neurodevelopmental and Psychiatric Disorders in Human iPSCSemantic proximity Read