Article

P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cells

2017-07-26

Abstract excerpt

<h4>SUMMARY</h4> CRISPR/Cas9 has revolutionized our ability to engineer genomes and to conduct genome-wide screens in human cells. While some cell types are easily modified with Cas9, human pluripotent stem cells (hPSCs) poorly tolerate Cas9 and are difficult to engineer. Using a stable Cas9 cell line or transient delivery of ribonucleoproteins (RNPs) we achieved an average insertion or deletion efficiency greate...

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Literature Corpus work: e3d4af30-66fa-50c7-893b-62d7943b683e
DOI: 10.1101/168443

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