Article

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus

2021-08-05

Abstract excerpt

<h4>ABSTRACT</h4> In recent years CRISPR-Cas9 knockouts (KO) have become increasingly ultilised to study gene function. MicroRNAs (miRNAs) are short non-coding RNAs, 20-22 nucleotides long, which affect gene expression through post-transcriptional repression. We previously identified miRNAs-196a and −219 as implicated in the development of Xenopus neural crest (NC). The NC is a multipotent stem-cell population,...

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Literature Corpus work: f60b1a5a-ee87-59ce-bbc0-4cc0b9152fef
DOI: 10.1101/2021.08.05.454468

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