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Article

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus.

Developmental biology - 1 Mar 2022

Godden Alice M, Antonaci Marco, Ward Nicole J, van der Lee Michael, Abu-Daya Anita, Guille Matthew, Wheeler Grant N

Abstract excerpt

In recent years CRISPR-Cas9 knockouts (KO) have become increasingly ultilised to study gene function. MicroRNAs (miRNAs) are short non-coding RNAs, 20-22 nucleotides long, which affect gene expression through post-transcriptional repression. We previously identified miRNAs-196a and -219 as implicated in the development of Xenopus neural crest (NC). The NC is a multipotent stem-cell population, specified during...

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Identifiers

PMID: 34968443
DOI: 10.1016/j.ydbio.2021.12.015
PMCID: PMC8865746

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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