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Article

A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.

Nature methods - 1 Jun 2020

Zuo Erwei, Sun Yidi, Yuan Tanglong, He Bingbing, Zhou Changyang, Ying Wenqin, Liu Jing, Wei Wu, Zeng Rong, Li Yixue, Yang Hui

Abstract excerpt

Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE...

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PMID: 32424272
DOI: 10.1038/s41592-020-0832-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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