Article
Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity.
Molecular cell - 3 Sept 2020
Jin Shuai, Fei Hongyuan, Zhu Zixu, Luo Yingfeng, Liu Jinxing, Gao Shenghan, Zhang Feng, Chen Yu-Hang, Wang Yanpeng, Gao Caixia
Abstract excerpt
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to mimic actively transcribed genomic loci that are more susceptible to cytidine deaminase, we set up a...
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