Article
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nature biotechnology - 1 May 2020
Doman Jordan L, Raguram Aditya, Newby Gregory A, Liu David R
Abstract excerpt
Cytosine base editors (CBEs) enable targeted C•G-to-T•A conversions in genomic DNA. Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide Cas9-independent off-target C•G-to-T•A mutation in mouse embryos and in rice. Here we develop multiple rapid, cost-effective methods to screen the propensity of different CBEs to induce Cas9-independent deamination in Escherichia coli and in...
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