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Article

Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA.

mBio - 20 Apr 2021

Hu Zhiwei, Wang Yannan, Liu Qian, Qiu Yan, Zhong Zhiyu, Li Kangdi, Li Wenhua, Deng Zixin, Sun Yuhui

Abstract excerpt

Base editing is a powerful genome editing approach that enables single-nucleotide changes without double-stranded DNA breaks (DSBs). However, off-target effects as well as other undesired editings at on-target sites remain obstacles for its application. Here, we report that bubble hairpin single guide RNAs (BH-sgRNAs), which contain a hairpin structure with a bubble region on the 5' end of the guide sequence, can...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33879582
DOI: 10.1128/mBio.00342-21
PMCID: PMC8092237

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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