Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.

Nature communications - 11 Nov 2020

Yesbolatova Aisha, Saito Yuichiro, Kitamoto Naomi, Makino-Itou Hatsune, Ajima Rieko, Nakano Risako, Nakaoka Hirofumi, Fukui Kosuke, Gamo Kanae, Tominari Yusuke, Takeuchi Haruki, Saga Yumiko, Hayashi Ken-Ichiro, Kanemaki Masato T

Abstract excerpt

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes it possible to observe an immediate phenotype. However, the current AID system has two major drawbacks: leaky degradation and the requirement for a high dose of auxin. These negative features make it difficult to control precisely the expression...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to HCT116 Cells
Share to Hippocampus

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Indoleacetic Acids

Share to Minichromosome Maintenance Proteins

Share to Proteolysis

Share to Proteomics

Share to Xenograft Model Antitumor Assays

Source

Open in PubMed Open full text

Identifiers

PMID: 33177522
DOI: 10.1038/s41467-020-19532-z
PMCID: PMC7659001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.PMID 33177522Read ↗

A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pairSemantic proximity Read ↗

Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinoleSemantic proximity Read ↗

One-step generation of auxin-inducible degron cells with high-efficiency homozygous taggingSemantic proximity Read ↗

HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cellsSemantic proximity Read ↗

Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cellsSemantic proximity Read ↗

Deciphering acquired resistance mechanisms to sustained auxin-inducible protein degradation in cells and miceSemantic proximity Read ↗

Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology DonorsSemantic proximity Read ↗

Comparative analysis and directed protein evolution yield an improved degron technology with minimal basal degradation, rapid inducible depletion, and faster recovery of target proteins.Semantic proximity Read ↗

The mIAA7 degron improves auxin-mediated degradation in <i>C. elegans</i>Semantic proximity Read ↗

Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID).Semantic proximity Read ↗

Engineering the auxin-inducible degron system for tunable <i>in vivo</i> control of organismal physiologySemantic proximity Read ↗

Efficacy of auxin-inducible protein degradation in <i>C. elegans</i> tissues using different auxins and TIR1-expressing strainsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.