Article

A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair

2020-01-20

Abstract excerpt

A uxin- I nducible D egron (AID) technology enables conditional depletion of targeted proteins. However, the applicability of the AID in vertebrate cells has been limited due to cytotoxicity caused by high auxin concentrations. Here, we establish an improved AID system using an engineered orthogonal auxin-TIR1 pair, which exhibits over 1,000 times stronger binding. With ~1,000-fold less auxin concentration, we a...

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Literature Corpus work: 8d3439ab-c9ac-5170-bd25-b4866993b878
DOI: 10.1101/2020.01.20.912113

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