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HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells

26 Feb 2024

Abstract excerpt

Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation of human AID cell lines is challenging, especially in human embryonic stem cells (hESCs). Here, we develop HiHo-AID2, a streamlined procedure for rapid, one-step generation of human cancer and hESC lines with high homozygous degron-tagging efficiency based on an...

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PMID: 38409044
DOI: 10.1186/s13059-024-03187-w

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