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Efficacy of auxin-inducible protein degradation in <i>C. elegans</i> tissues using different auxins and TIR1-expressing strains

2024-01-20

Abstract excerpt

<h4>Summary</h4> The auxin-inducible degradation system has emerged as a powerful tool to deplete proteins of interest in cells and tissues of various model organisms, including C. elegans 2–5 . Here, we present a detailed protocol to perform AID-driven spatiotemporal depletion of specific proteins in C. elegans tissues. First, we introduced the AID degron and a fluorescent reporter at two conserved proteins: (...

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Literature Corpus work
69c844fa-71cd-5532-9adf-588e48e6b893
DOI
10.1101/2024.01.16.575916
Open publication

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Efficacy of auxin-inducible protein degradation in <i>C. elegans</i> tissues using different auxins and TIR1-expressing strainsDOI 10.1101/2024.01.16.575916
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