Article

Near 100% genome editing efficiency with a one-step all RNA prime editing system in a human reporter iPSC line

2024-12-21

Abstract excerpt

CRISPR/Cas9 nucleases offer powerful tools for genome editing but can cause unintended mutations at the target site due to the creation of double-stranded DNA breaks. Prime editing (PE) is arguably a safer technology as it relies on the creation of single-strand DNA nicks and avoids possible indels. Despite its precision, current PE systems are limited by relatively low editing efficiency in non-immortalized cells...

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Literature Corpus work: eda56490-c898-5124-9f35-21fd5e0d1fba
DOI: 10.1101/2024.12.20.629762

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