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Article

A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.

Scientific reports - 12 Nov 2021

Eggenschwiler Reto, Gschwendtberger Thomas, Felski Christian, Jahn Christopher, Langer Florian, Sterneckert Jared, Hermann Andreas, Lühmann Jonathan, Steinemann Doris, Haase Alexandra, Martin Ulrich, Petri Susanne, Cantz Tobias

Abstract excerpt

CRISPR prime-editors are emergent tools for genome editing and offer a versatile alternative approach to HDR-based genome engineering or DNA base-editors. However, sufficient prime-editor expression levels and availability of optimized transfection protocols may affect editing efficiencies, especially in hard-to-transfect cells like hiPSC. Here, we show that piggyBac prime-editing (PB-PE) allows for sustained...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34773059
DOI: 10.1038/s41598-021-01689-2
PMCID: PMC8589839

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