Article

Enrichment of allelic editing outcomes by Prime Editing in iPS cells

2024-03-19

Abstract excerpt

Gene editing in human induced pluripotent stem (iPS) cells with programmable nucleases facilitates reliable disease models, but methods using double-strand break repair often produce random on-target by-products. Prime editing (PE) combines Cas9 nickase with reverse transcriptase (RT) and a prime editing guide RNA (pegRNA) encoding a repair template to reduce by-products. We implemented a GMP-compatible protocol f...

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Literature Corpus work: b0b7c0af-c73b-57a0-a52a-9db5ff7f571d
DOI: 10.1101/2024.03.19.585665

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