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Article

Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells.

The CRISPR journal - 1 Oct 2024

Niwa Ryo, Matsumoto Tomoko, Liu Alexander Y, Kawato Maki, Kondo Takayuki, Tsukita Kayoko, Gee Peter, Inoue Haruhisa, Maurissen Thomas L, Woltjen Knut

Abstract excerpt

Gene editing in human induced pluripotent stem (iPS) cells with programmable nucleases facilitates reliable disease models, but methods using double-strand break repair often produce random on-target by-products. Prime editing (PE) combines Cas9 nickase with reverse transcriptase and PE guide RNA (pegRNA) encoding a repair template to reduce by-products. We implemented a GMP-compatible protocol for transfecting...

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Identifiers

PMID: 39436282
DOI: 10.1089/crispr.2024.0028

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