Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors.

Genes - 6 May 2020

Sürün Duran, Schneider Aksana, Mircetic Jovan, Neumann Katrin, Lansing Felix, Paszkowski-Rogacz Maciej, Hänchen Vanessa, Lee-Kirsch Min Ae, Buchholz Frank

Abstract excerpt

In contrast to CRISPR/Cas9 nucleases, CRISPR base editors (BE) and prime editors (PE) enable predefined nucleotide exchanges in genomic sequences without generating DNA double strand breaks. Here, we employed BE and PE mRNAs in conjunction with chemically synthesized sgRNAs and pegRNAs for efficient editing of human induced pluripotent stem cells (iPSC). Whereas we were unable to correct a disease-causing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Aminohydrolases
Share to Autoimmune Diseases of the Nervous System

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Associated Protein 9

Share to Cytosine

Share to Embryoid Bodies

Share to Nervous System Malformations

Share to RNA, Guide, CRISPR-Cas Systems

Share to SAM Domain and HD Domain-Containing Protein 1

Source

Open in PubMed Open full text

Identifiers

PMID: 32384610
DOI: 10.3390/genes11050511
PMCID: PMC7288465

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors.PMID 32384610Read ↗

Comprehensive analysis of prime editing outcomes in human embryonic stem cellsSemantic proximity Read ↗

Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells.Semantic proximity Read ↗

A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cellsSemantic proximity Read ↗

Precise and efficient scarless genome editing in stem cells using CORRECT.Semantic proximity Read ↗

Highly efficient generation of isogenic pluripotent stem cell models using prime editingSemantic proximity Read ↗

Enhancing Precise Genome Editing in Human Pluripotent Stem Cells through Dual Inhibition of DNA Damage Response and Repair PathwaysSemantic proximity Read ↗

Prime editing: A gene precision editing tool from inception to present.Semantic proximity Read ↗

Evaluating CRISPR-based Prime Editing for cancer modeling and CFTR repair in intestinal organoidsSemantic proximity Read ↗

Precision genome-editing with CRISPR/Cas9 in human induced pluripotent stem cellsSemantic proximity Read ↗

Gene editing with 'pencil' rather than 'scissors' in human pluripotent stem cells.Semantic proximity Read ↗

Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells.Semantic proximity Read ↗

Enrichment of allelic editing outcomes by Prime Editing in iPS cellsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.