Article

Highly efficient generation of isogenic pluripotent stem cell models using prime editing

2022-02-15

Abstract excerpt

<h4>SUMMARY</h4> The recent development of prime editing (PE) genome engineering technologies has the potential to significantly simplify the generation of human pluripotent stem cell (hPSC)-based disease models. PE is a multi-component editing system that uses a Cas9-nickase fused to a reverse transcriptase (nCas9-RT) and an extended PE guide RNA (pegRNA). Once reverse transcribed, the pegRNA extension functions...

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Literature Corpus work: 3b2059fb-a548-5e23-9fcc-7947cf7a9822
DOI: 10.1101/2022.02.15.480601

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