Article

Efficient and accurate prime editing strategy to correct genetic alterations in hiPSC using single EF-1alpha driven all-in-one plasmids

2022-05-04

Abstract excerpt

<h4>ABSTRACT</h4> Prime editing (PE) is currently the most effective and versatile technology to make targeted alterations in the genome. Several improvements to the PE machinery have recently been made, and have been tested in a range of model systems, including immortalized cell lines, stem-cells and animal models. While nick RNA (ncRNA)-dependent PE systems like PE3 and PE5 are currently considered to be the m...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bf586322-5f46-5637-97aa-99e7d77694d7
DOI: 10.1101/2022.05.04.490422

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Efficient and accurate prime editing strategy to correct genetic alterations in hiPSC using single EF-1alpha driven all-in-one plasmidsDOI 10.1101/2022.05.04.490422Read

A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.Semantic proximity Read

Enrichment of allelic editing outcomes by Prime Editing in iPS cellsSemantic proximity Read

Highly efficient generation of isogenic pluripotent stem cell models using prime editingSemantic proximity Read

Near 100% genome editing efficiency with a one-step all RNA prime editing system in a human reporter iPSC lineSemantic proximity Read

Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells.Semantic proximity Read

Programming large target genomic deletion and concurrent insertion via a prime editing-based method: PEDARSemantic proximity Read

Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases.Semantic proximity Read

Optimized nickase- and nuclease-based prime editing in human and mouse cellsSemantic proximity Read

TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single- and Multi-Locus Genome EngineeringSemantic proximity Read

TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome EngineeringSemantic proximity Read

Mutation-specific reporter for optimization and enrichment of prime editing.Semantic proximity Read

Marker-free coselection for successive rounds of prime editing in human cellsSemantic proximity Read