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Article

Synthetic chimeric nucleases function for efficient genome editing.

Nature communications - 4 Dec 2019

Liu R M, Liang L L, Freed E, Chang H, Oh E, Liu Z Y, Garst A, Eckert C A, Gill R T

Abstract excerpt

CRISPR-Cas systems have revolutionized genome editing across a broad range of biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and engineered for improved capabilities. Given the modular structure of such enzymes, we hypothesized that engineering chimeric sequences would generate non-natural variants that span the kinetic parameter landscape, and thus provide for the rapid selection of...

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Identifiers

PMID: 31797930
DOI: 10.1038/s41467-019-13500-y
PMCID: PMC6892893

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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