Article

CRISPR-COPIES: An <i>in silico</i> platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration

2023-09-06

Abstract excerpt

The CRISPR/Cas system has emerged as a powerful tool for genome editing in metabolic engineering and human gene therapy. However, locating the optimal site on the chromosome to integrate heterologous genes using the CRISPR/Cas system remains an open question. Selecting a suitable site for gene integration involves considering multiple complex criteria, including factors related to CRISPR/Cas-mediated integration,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 86b53893-a046-5862-9437-27728fd6b376
DOI: 10.1101/2023.09.06.556564

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-COPIES: An <i>in silico</i> platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integrationDOI 10.1101/2023.09.06.556564Read

Expanding the CRISPR toolbox by engineering Cas12a orthologs of metagenomic discoverySemantic proximity Read

Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrasesSemantic proximity Read

Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA).Semantic proximity Read

Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposasesSemantic proximity Read

Improving FnCas12a genome editing by exonuclease fusionSemantic proximity Read

Target-specific precision of CRISPR-mediated genome editingSemantic proximity Read

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs.Semantic proximity Read

A catalog of gene editing sites and genetic variations in editing sites in model organisms.Semantic proximity Read

Massively Parallel Profiling of RNA-targeting CRISPR-Cas13dSemantic proximity Read

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target SitesSemantic proximity Read

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell eliminationSemantic proximity Read

CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsSemantic proximity Read