Article

Rational engineering of allosteric protein switches by <i>in silico</i> prediction of domain insertion sites

2024-12-04

Abstract excerpt

Domain insertion engineering is a powerful approach to juxtapose otherwise separate biological functions, resulting in proteins with new-to-nature activities. A prominent example are switchable protein variants, created by receptor domain insertion into effector proteins. Identifying suitable, allosteric sites for domain insertion, however, typically requires extensive screening and optimization. We present ProDom...

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Literature Corpus work: 6df6e2e2-a176-5660-b7fc-fe85a361e7f6
DOI: 10.1101/2024.12.04.626757

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