Article

Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation

2025-08-08

Abstract excerpt

Precise regulation of transcription factor (TF) expression is critical for maintaining cell identity, but studies on how graded expression levels affect cellular phenotypes are limited. To address this gap, we employed human embryonic stem cells (hESCs) as a dynamic model to study gene dosage effects and systematically titrated key TFs NANOG and OCT4 expression using CRISPR interference (CRISPRi). We then profil...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e9639794-47ea-5210-8c98-e37e3ff47ebe
DOI: 10.1101/2025.08.07.669196

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiationDOI 10.1101/2025.08.07.669196Read

Transcription factor heterogeneity in pluripotent stem cells: a stochastic advantage.Semantic proximity Read

DoseH-seq: A single-cell multiome platform to decode gene-dosage logic driving developmental reversion and cell fate reprogrammingSemantic proximity Read

Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identitySemantic proximity Read

Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells.Semantic proximity Read

Pluripotency Factors Modulate Interferon Signaling in Embryonic Stem CellsSemantic proximity Read

Single-cell dynamics of core pluripotency factors in human pluripotent stem cellsSemantic proximity Read

Mapping dynamic histone acetylation patterns to gene expression in nanog-depleted murine embryonic stem cells.Semantic proximity Read

Inference of gene regulatory networks using time-series single-cell RNA-seq data with CRISPR perturbationsSemantic proximity Read

Low-level repressive histone marks fine-tune stemness gene transcription in neural stem cellsSemantic proximity Read

Nonlinear transcriptional responses to gradual modulation of transcription factor dosageSemantic proximity Read

Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotencySemantic proximity Read

Comparative benchmarking of CRISPRi and CasRx in standardized pluripotent stem cell platforms reveals context-dependent knockdown performanceSemantic proximity Read