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New design strategies for ultra-specific CRISPR-Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivity

2023-07-28

Abstract excerpt

<h4>ABSTRACT</h4> The pressing need for clinical diagnostics has required the development of novel nucleic acid-based detection technologies that are sensitive, fast, and inexpensive, and that can be deployed at point-of-care. Recently, the RNA-guided ribonuclease CRISPR-Cas13 has been successfully harnessed for such purposes. However, developing assays for detection of genetic variability, for example single-nuc...

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Literature Corpus work
10d8c3af-61c9-5e57-b412-0a168724439a
DOI
10.1101/2023.07.26.550755
Open publication

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New design strategies for ultra-specific CRISPR-Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivityDOI 10.1101/2023.07.26.550755
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