Article

Mechanism of human Lig1 regulation by PCNA in Okazaki fragment sealing

2022-02-22

Abstract excerpt

<title>Abstract</title> <p>During lagging strand synthesis, DNA Ligase 1 (Lig1) cooperates with the sliding clamp PCNA to seal the nicks between Okazaki fragments generated by Flap endonuclease 1 (FEN1), but the structural basis of this mechanism is unknown. We present cryo-EM structures of human Lig1 bound to nicked DNA and PCNA in absence and presence of FEN1. Lig1 and PCNA form a two-ring complex encircling th...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e2cd85d1-1a44-53b8-902c-0ae9048c1a87
DOI: 10.21203/rs.3.rs-1323117/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Mechanism of human Lig1 regulation by PCNA in Okazaki fragment sealingDOI 10.21203/rs.3.rs-1323117/v1Read

Structures of LIG1 uncover a lack of sugar discrimination against a ribonucleotide at the 3'-end of nick DNASemantic proximity Read

Structural and biochemical characterization of LIG1 during mutagenic nick sealing of oxidatively damaged ends at the final step of DNA repairSemantic proximity Read

Characterization of nick binding and sealing by LIG1 Huntington’s disease-asssociated K845N variant at biochemical, structural, and single-molecule levelsSemantic proximity Read

DNA clamp function of the mono-ubiquitinated Fanconi Anemia FANCI-FANCD2 complexSemantic proximity Read

Processing of branched DNA intermediates by a complex of human FEN-1 and PCNASemantic proximity Read

Human DNA ligase I efficiently seals nicks in nucleosomes.Semantic proximity Read

LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency.Semantic proximity Read

XPF mediates 3’ flap processing for FEN1-independent Okazaki fragment maturationSemantic proximity Read

High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication.Semantic proximity Read

XPF mediates 3' flap processing for FEN1-independent Okazaki fragment maturation.Semantic proximity Read

Structures of DNA-bound human ligase IV catalytic core reveal insights into substrate binding and catalysis.Semantic proximity Read

DNA Ligases: Progress and ProspectsSemantic proximity Read