Article

Characterization of nick binding and sealing by LIG1 Huntington’s disease-asssociated K845N variant at biochemical, structural, and single-molecule levels

2025-04-26

Abstract excerpt

DNA ligase 1 (LIG1) joins broken strand breaks and discriminates against nicks containing mismatch or oxidative damage. Huntington’s disease (HD)-associated mutation K845N in LIG1 gene has been predicted to be onset delaying and suppresses CAG repeat expansion. Yet, how this mutation impacts faithful nick sealing and efficient DNA binding by LIG1 remains unknown. Here, using biochemistry, X-ray crystallography, a...

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Literature Corpus work: d44658ee-20cc-5874-a6e6-e6905f59876f
DOI: 10.1101/2025.04.26.650789

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Characterization of nick binding and sealing by LIG1 Huntington’s disease-asssociated K845N variant at biochemical, structural, and single-molecule levelsDOI 10.1101/2025.04.26.650789Read

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