Article

Early Reprogramming Intermediates Enable Direct Neuronal Conversion via NGN2

2025-09-22

Abstract excerpt

<title>Abstract</title> <p>The generation of engineered neurons via Neurogenin-2 (NGN2) overexpression, starting from human induced pluripotent stem cells (hiPSCs), is a powerful tool for modeling neurological diseases. However, using stabilized hiPSCs as a starting point significantly increases the time required to obtain a valuable human neuronal model in vitro. Here, we demonstrated that as little as 3 days of...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e1e1993f-5c95-5471-92c4-7c1ae19afc8e
DOI: 10.21203/rs.3.rs-7294789/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Early Reprogramming Intermediates Enable Direct Neuronal Conversion via NGN2DOI 10.21203/rs.3.rs-7294789/v1Read

Early Reprogramming Intermediates Enable Direct Neuronal Conversion via NGN2Semantic proximity Read

Ngn2 induces diverse neuronal lineages from human pluripotencySemantic proximity Read

Rapid, Growth Factor-Reduced Differentiation of Functional Neurons from hiPSCsSemantic proximity Read

Integrative genomic analysis of Neurogenin2 reprogramming of human iPSCsSemantic proximity Read

A Snapshot of Early Transcriptional Changes Accompanying the Pro-Neural Phenotype Switch by NGN2, ASCL1, SOX2, and MSI1 in Human Fibroblasts: An RNA-Seq Study.Semantic proximity Read

Direct reprogramming of human somatic cells into neuron-like cells involves a transition through a transient intermediate stateSemantic proximity Read

Insights for disease modeling from single cell transcriptomics of iPSC-derived Ngn2-induced neurons and astrocytes across differentiation time and co-cultureSemantic proximity Read

Human cortical neurons rapidly generated by direct ES cell programming integrate into stroke-injured rat cortexSemantic proximity Read

Concise Review: Generation of Neurons From Somatic Cells of Healthy Individuals and Neurological Patients Through Induced Pluripotency or Direct ConversionSemantic proximity Read

Expandable and Rapidly Differentiating Human Induced Neural Stem Cell Lines for Multiple Tissue Engineering Applications.Semantic proximity Read

Comprehensive Characterization of the Human Neural Stem Cell Line HNSC.100 as a Versatile Model for Neurobiological ResearchSemantic proximity Read

Proliferation history and transcription factor levels drive direct conversionSemantic proximity Read