Article

Rapid, Growth Factor-Reduced Differentiation of Functional Neurons from hiPSCs

2025-12-03

Abstract excerpt

<h4>Introduction</h4> Human induced pluripotent stem cells (hiPSCs) can be rapidly converted into neurons via NGN2 overexpression, but many protocols require costly reagents during the initial induction phase that may limit adoption by labs without routine neuronal differenitation experience. We developed a simplified, low-cost protocol using a tetracyline-inducible (TET-on) NGN2 system in minimal media to genera...

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Literature Corpus work: 35470798-2810-5b4c-8b7d-ed8fced83d94
DOI: 10.64898/2025.12.03.692194

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