Article

Oxford Nanopore sequencing-based protocol to detect CpG methylation in human mitochondrial DNA

2021-02-20

Abstract excerpt

<h4>Summary</h4> Methylation on CpG residues is one of the most important epigenetic modifications of nuclear DNA, regulating gene expression. Methylation of mitochondrial DNA (mtDNA) has been studied using whole genome bisulfite sequencing (WGBS), but recent evidence has uncovered major technical issues which introduce a potential bias during methylation quantification. Here, we validate the technical concerns w...

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Literature Corpus work: df63bad4-9ae9-5204-b3f3-3fc78b2891cd
DOI: 10.1101/2021.02.20.432086

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