Article

Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64

2024-10-10

Abstract excerpt

E-64 is an irreversible and selective cysteine protease inhibitor prominently used in chemical biology and drug discovery. In this work, we uncovered and characterized the NRPS-independent pathway responsible for biosynthesis of E-64, which is widely conserved in fungi. Heterologous reconstitution and biochemical assays show the pathway starts with epoxidation of fumaric acid to the warhead (2 S ,3 S )- trans -...

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Literature Corpus work: dae610f4-553b-5019-b99f-927982b167dd
DOI: 10.1101/2024.10.09.617497

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