Article

High-throughput engineering and modification of non-ribosomal peptide synthetases based on Golden Gate assembly

2025-04-24

Abstract excerpt

Non-ribosomal peptide synthetases (NRPS) are multimodular enzymes that produce complex peptides with diverse biological activities, potentially being used as clinical drugs. However, the pharmaceutical applications of such natural peptides often require further derivatisation and modification of the peptide backbone, mainly performed by chemical synthesis. A sustainable alternative resembles the in vivo engineeri...

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Identifiers and source

Literature Corpus work: 177096df-8146-53ee-8a1e-52e09ccf2858
DOI: 10.1101/2025.04.23.650154

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