Article

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

2022-03-16

Abstract excerpt

Nucleases derived from the prokaryotic defense system CRISPR-Cas are frequently re-purposed for gene editing and molecular diagnostics. Hence, an in-depth understanding of the molecular mechanisms of these enzymes is of crucial importance. We focused on Cas12a from Francisella novicida (FnCas12a) and investigated the functional role of helix 1, a structural element that together with the bridge helix (BH) connect...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d935f395-c663-5b34-b59a-0665a8625519
DOI: 10.1101/2022.03.15.484427

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domainDOI 10.1101/2022.03.15.484427Read

Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions.Semantic proximity Read

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.Semantic proximity Read

Engineering salt-tolerant Cas12f1 variants for gene-editing applications.Semantic proximity Read

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavageSemantic proximity Read

Comparative Structural and Dynamics Study of Free and gRNA-bound FnCas9 and SpCas9 ProteinsSemantic proximity Read

Catalytically inactive dKbCas12d guided by sgRNA and new insights into its binding through a single-molecule approachSemantic proximity Read

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a.Semantic proximity Read

Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavageSemantic proximity Read

Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnosticsSemantic proximity Read

Differential Allosteric Modulation of Cas9 Specificity.Semantic proximity Read

Structural insights into DNA cleavage activation of CRISPR-Cas9 system.Semantic proximity Read

Essential Structural and Functional Roles of the Cmr4 Subunit in RNA Cleavage by the Cmr CRISPR-Cas ComplexSemantic proximity Read