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Article

Differential Allosteric Modulation of Cas9 Specificity.

Journal of chemical theory and computation - 27 Jan 2026

Li Yuanhao, Li Xin, Chen Yingjie, Wang Yanqing, Zuo Zhicheng

Abstract excerpt

Both RNA- and protein-based strategies have been developed to mitigate off-target cleavage by CRISPR-Cas9, yielding noncanonical guide RNAs (gRNAs) and Cas9 variants with enhanced gene-editing precision. However, the molecular mechanisms by which such PAM-distal alterations─remote from the nuclease centers─modulate Cas9 activity and specificity remain incompletely understood. Here, we performed near-millisecond...

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Identifiers

PMID: 41511442
DOI: 10.1021/acs.jctc.5c01919

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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