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Article

Structural insights into DNA cleavage activation of CRISPR-Cas9 system.

Nature communications - 9 Nov 2017

Huai Cong, Li Gan, Yao Ruijie, Zhang Yingyi, Cao Mi, Kong Liangliang, Jia Chenqiang, Yuan Hui, Chen Hongyan, Lu Daru, Huang Qiang

Abstract excerpt

CRISPR-Cas9 technology has been widely used for genome engineering. Its RNA-guided endonuclease Cas9 binds specifically to target DNA and then cleaves the two DNA strands with HNH and RuvC nuclease domains. However, structural information regarding the DNA cleavage-activating state of two nuclease domains remains sparse. Here, we report a 5.2 Å cryo-EM structure of Cas9 in complex with sgRNA and target DNA. This...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29123204
DOI: 10.1038/s41467-017-01496-2
PMCID: PMC5680257

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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