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Article

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.

Biochemistry - 14 Dec 2021

Babu Kesavan, Kathiresan Venkatesan, Kumari Pratibha, Newsom Sydney, Parameshwaran Hari Priya, Chen Xiongping, Liu Jin, Qin Peter Z, Rajan Rakhi

Abstract excerpt

CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection in bacteria and archaea against intruding genomic materials. Cas9, a type-II CRISPR effector protein, is widely used for gene editing applications since a single guide RNA can direct Cas9 to cleave specific genomic targets. The conformational changes associated with RNA/DNA binding are being modulated to develop Cas9 variants with...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Cleavage

Share to Protein Conformation, alpha-Helical

Share to Protein Conformation, beta-Strand

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Source

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Identifiers

PMID: 34757726
DOI: 10.1021/acs.biochem.1c00354
PMCID: PMC8675354

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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