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Article

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a.

Nucleic acids research - 5 Jan 2026

Newman Anthony, Starrs Lora, Burgio Gaetan

Abstract excerpt

The trans cleavage activity of Cas12a has been extensively used for the detection of biomolecules. Different Cas12a orthologues exhibit faster or slower trans cleavage kinetics, making some orthologues more suited for sensitive molecular detection. Ionic strength of reaction buffers and mutations that change the electrostatic environment near the RuvC active site have also been reported to strongly influence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41521661
DOI: 10.1093/nar/gkaf1485
PMCID: PMC12784958

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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