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Article

Engineering salt-tolerant Cas12f1 variants for gene-editing applications.

Journal of biomolecular structure & dynamics - 1 Sept 2024

Daskalakis Vangelis, Papapetros Spyridon

Abstract excerpt

CRISPR has revolutionized the field of genome editing in life sciences by serving as a versatile and state-of-the-art tool. Cas12f1 is a small nuclease of the bacterial immunity CRISPR system with an ideal size for cellular delivery, in contrast to CRISPR-associated (Cas) proteins like Cas9 or Cas12. However, Cas12f1 works best at low salt concentrations. In this study, we find that the plasticity of certain...

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Identifiers

PMID: 37526217
DOI: 10.1080/07391102.2023.2240418

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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