Article

Comparative Structural and Dynamics Study of Free and gRNA-bound FnCas9 and SpCas9 Proteins

2022-03-30

Abstract excerpt

The introduction of CRISPR/Cas9 based gene editing has greatly accelerated therapeutic genome editing. However, the off-target DNA cleavage by CRISPR/Cas9 protein hampers its clinical translation, thereby hindering its widespread use as a programmable genome editing tool. Although Cas9 variants with better mismatch discrimination have been developed, they have significantly lower rates of on-target DNA cleavage. H...

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Literature Corpus work: 53173821-de10-598d-911d-39dc49936f2d
DOI: 10.1101/2022.03.30.486385

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