Article

Catalytically inactive dKbCas12d guided by sgRNA and new insights into its binding through a single-molecule approach

2023-12-12

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas12d is a distinct V-D type system discovered in the metagenomes of Candidate Phyla Radiation bacteria. It stands out from most closely related systems due to its 17-19 nucleotide short spacer region and specialized stabilizing scoutRNAs. We made significant improvements to this system by modifying its scoutRNA to create sgRNA, which greatly simplifies its use. We found mutations in the...

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Literature Corpus work: 19a3cd7f-ab7e-513d-ac6e-c36c5700a561
DOI: 10.1101/2023.12.12.571249

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