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Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage

30 Dec 2024

Abstract excerpt

Exceptionally diverse type V CRISPR-Cas systems provide numerous RNA-guided nucleases as powerful tools for DNA manipulation. Two known Cas12e nucleases, DpbCas12e and PlmCas12e, are both effective in genome editing. However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e’s enzymatic properties. To comprehensively understand the Cas12e family, we identify...

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CRISPR and Genetic Engineering
Mosquito-borne diseases and control
RNA regulation and disease

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 39737904
DOI: 10.1038/s41467-024-54491-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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