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Article

Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein.

Stem cell reports - 13 Apr 2021

Kagita Akihiro, Lung Mandy S Y, Xu Huaigeng, Kita Yuto, Sasakawa Noriko, Iguchi Takahiro, Ono Miyuki, Wang Xiou H, Gee Peter, Hotta Akitsu

Abstract excerpt

Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin frequency is low compared with deletion induction. Although several Cas9 transduction methods have been reported, the biochemical behavior of CRISPR-Cas9 nuclease in...

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Identifiers

PMID: 33711268
DOI: 10.1016/j.stemcr.2021.02.013
PMCID: PMC8072016

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Source receipt: pubmed, retrieved 7 Sept 2026.

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