Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein.

doi:10.1016/j.stemcr.2021.02.013

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Article

Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein.

Stem cell reports - 13 Apr 2021

Kagita Akihiro, Lung Mandy S Y, Xu Huaigeng, Kita Yuto, Sasakawa Noriko, Iguchi Takahiro, Ono Miyuki, Wang Xiou H, Gee Peter, Hotta Akitsu

Abstract excerpt

Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin frequency is low compared with deletion induction. Alth...

Topics

Alleles
Anti-Bacterial Agents
Base Sequence
CRISPR-Associated Protein 9
CRISPR-Cas Systems
Distal Myopathies
Dysferlin
Exons
Gene Editing
HEK293 Cells
Haplotypes
Homozygote
Humans
Induced Pluripotent Stem Cells

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Muscular Atrophy

Muscular Dystrophy, Duchenne

Mutagenesis, Insertional

Mutation

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Identifiers

PMID: 33711268
DOI: 10.1016/j.stemcr.2021.02.013
PMCID: PMC8072016

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-33711268-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.