Programmable downsizing of CRISPR-Cas9 activity for precise and safe genome editing

Abstract excerpt

CRSIPR-Cas9 system has opened up the avenue to efficient genome editing 1–4 . However, together with known off-target effects, several concerns of current CRISPR-Cas9 platform, including severe DNA damage, cytotoxicity, and large genomic alteration, have emerged in recent reports 5–7 and establish a formidable obstacle to precisely model allele dosage effects of disease mutations and risk variants, especially mo...

Topics

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• CRISPR and Genetic Engineering
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Gene Editing
• Genome, Human
• Mammals
• Mutation
• Zebrafish

Identifiers and source

Literature Corpus work

748752c1-f660-5490-ab26-2118bb293eaa

DOI

10.1101/2020.10.31.361733