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Article

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency.

Scientific reports - 24 Jun 2016

Zhang Jian-Ping, Li Xiao-Lan, Neises Amanda, Chen Wanqiu, Hu Lin-Ping, Ji Guang-Zhen, Yu Jun-Yao, Xu Jing, Yuan Wei-Ping, Cheng Tao, Zhang Xiao-Bing

Abstract excerpt

CRISPR-Cas9 is a powerful genome editing technology, yet with off-target effects. Truncated sgRNAs (17nt) have been found to decrease off-target cleavage without affecting on-target disruption in 293T cells. However, the potency of 17nt sgRNAs relative to the full-length 20nt sgRNAs in stem cells, such as human mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), has not been assessed. Using...

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PMID: 27338021
DOI: 10.1038/srep28566
PMCID: PMC4919781

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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